this is from the manual word for word......
"Fuel Requirements: your vehicle will operate on both unleaded regular gasoline with an octane rating of 87 AKI (Anti-Knock Index) number (research octane number 91), or E-85 fuel, or any mixture of these two. for the best starting and vehicle driveability results, do not continually switch between E-85 and unleaded regular gasoline. if you must switch fuels, NISSAN recommends the following:
1. do not switch when the fuel gauge indicates less than 1/4 full
2. add more than 5 gallons of fuel
3. operate the vehicle immediately after refueling for a period of at least 5 minutes.
When the ambient temperature is above 90 degrees Fahrenheit you may experience hard starting and rough idle following start-up even if the above recommendations are followed.
Starting: The characteristics of E-85 fuel make it unsuitable for use when ambient temperatures fall below 0 degrees. in the range of 0 degrees to 32 degrees you may experience an increase in the time it takes for your engine to start and a deterioration in driveability (sags and or hesitations) until the engine is fully warmed up.
Fuel Consumption: Because E-85 fuel contains less energy per gallon than gasoline, you will experience an increase in fuel consumption. You can expect your fuel mileage to DECREASE by about 30% compared to gasoline consumption.
Maintenance: If you operate your vehicle using E-85 fuel, follow the correct maintenance log shown in the *NISSAN Service and Maintenance guide."
this was word for word from the manual, i cant believe the 30% less mileage part lol!!!!!